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. 2020 Jun 4;48(13):7154–7168. doi: 10.1093/nar/gkaa474

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Epistasis relationships implicate Set2 and the Clr6-CII HDAC complex downstream of Cdk9 and H2Bub1. (A) Antisense transcript levels at cdc2⁺ and erg32⁺ loci were quantified by strand-specific RT-qPCR in the indicated strains. Error bars denote S.D. from 3–4 independent experiments; ‘*’ denotes significant differences (P< 0.05; paired t-test) between the indicated double mutant strain and the respective single mutants. Value for the wild-type strain was set to 1. (B) As in (A) for the indicated cdk9^as strains. ‘*’ denotes significant differences (P< 0.05; unpaired t-test) between 3-MB-PP1-treated cells and the corresponding DMSO-treated control. Value for the DMSO-treated cdk9^as strain was set to 1.