Figure 6.

Antisense induction in cdk9^as htb1-K119R correlates with increased histone acetylation and decreased histone occupancy. (A) ChIP-qPCR was performed with the indicated antibodies (top) and quantified with primer pairs at the indicated positions in cdc2⁺ (see Materials and Methods). ‘–‘ and ‘+’ denote treatment with DMSO or 3-MB-PP1, respectively. Error bars denote S.D. from 3–4 independent experiments. ‘*’ denotes a significant difference (P<0.05; unpaired t-test) between treatments (DMSO or 3-MB-PP1) for a given strain; ‘#’ denotes a significant difference between strains for a given treatment; ‘∼’ denotes significant differences in both comparisons. (B) As in (A) for erg32⁺.