Figure 5.

ASO-ψ increases apical membrane conductance. (A) Average Ieq traces were calculated from hBE cells isolated from a CF patient donor homozygous for CFTR c.3718-2477C>T (donor 3) treated with vehicle (grey line), ASO-C (black line) or ASO-ψ (green line). Average (B) apical conductance (G_a) and (C) basolateral conductance (G_b) were calculated by fitting impedance spectra data from the patient epithelial cells to equivalent circuit equations that model epithelial membranes after benzamil, forskolin, VX-770 and GlyH-101 additions. The four data points following the initial compound addition were averaged (±SEM, N = 2; two-way ANOVA; Tukey's multiple comparisons test between treatment groups, *P< 0.05, **P< 0.01, ****P< 0.0001; Sidak's multiple comparisons test within treatment groups, ^#P< 0.05).