Table 5.
Protein abundance within the milk fat globule membrane secreted by 10 primiparous (PP) and 10 multiparous (MP) Holsteins at four milkings postpartum (M1, M2, M4, M14) that were impacted by the interaction of milking and parity (MIL × PAR)
| Accession number | Description | M1 | M2 | M4 | M14 | P-value | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| PP | MP | SE1 | PP | MP | SE | PP | MP | SE | PP | MP | SE | |||
| G3MXB5 | Immunoglobulin IgA heavy chain constant region, partial | 61.6 | 159.2 | 18.8 | 68.2 | 197.3 | 19.5 | 16.6 | 122.9 | 11.9 | 15.1 | 37 | 6.7 | <.0001 |
| P81265 | Polymeric immunoglobulin receptor | 79.8 | 146 | 17.5 | 75.4 | 169.6 | 19 | 30.7 | 131.1 | 9.3 | 32.1 | 49.1 | 6.9 | <.0001 |
| Q3SYR8 | Immunoglobulin J chain | 89.6 | 159 | 12.4 | 85.5 | 138 | 11.1 | 40.1 | 112.6 | 6 | 37.4 | 48.4 | 6.7 | <.0001 |
| F1MH40 | IGK protein | 92.3 | 210.8 | 38.1 | 76.4 | 122.3 | 13 | 27.4 | 102.1 | 11.1 | 27.3 | 31.5 | 4.4 | 0.0001 |
| G3N2D7 | Immunoglobulin light chain, partial | 89.7 | 120.4 | 12.7 | 71.7 | 94.4 | 9.6 | 43.9 | 108.3 | 9.2 | 49.1 | 42.4 | 5.9 | 0.0002 |
| F1MCF8 | IGL@ protein | 92 | 201.6 | 48.9 | 72.4 | 100.5 | 13.9 | 21.4 | 90.6 | 10.5 | 18.6 | 23.6 | 3.8 | 0.0007 |
| Q0VCN9 | Folate receptor 2 (Fetal) | 139.8 | 160.9 | 15.6 | 124.3 | 119.6 | 19.4 | 59 | 97.3 | 6.1 | 47.1 | 39.5 | 4.3 | 0.0007 |
| F1N5M2 | Vitamin D-binding protein | 118.1 | 176.1 | 24.3 | 83.4 | 109.8 | 15.8 | 42.8 | 93.1 | 9.2 | 44 | 36 | 4.9 | 0.0008 |
| F1MHS5 | Protein S100-A9 | 68 | 162.5 | 37.6 | 69.6 | 149.9 | 23 | 38.6 | 161 | 23 | 30.8 | 33.2 | 7.5 | 0.0011 |
| P24627 | Lactotransferrin | 54 | 182 | 45.9 | 53 | 100.2 | 13.8 | 41.2 | 109.8 | 12 | 42.7 | 44.7 | 6.1 | 0.0014 |
| Q1JPB0 | Leukocyte elastase inhibitor | 85 | 180.7 | 32.8 | 67.2 | 118.8 | 14.3 | 49.2 | 122.8 | 12.2 | 47.3 | 46.1 | 8.7 | 0.0024 |
| G5E5V1 | Immunoglobulin iota chain-like, partial (TPA) | 95.1 | 175.5 | 29.6 | 75.2 | 112.8 | 11.4 | 29.6 | 100.6 | 12.6 | 28.9 | 28.8 | 7 | 0.0027 |
| P28782 | Protein S100-A8 | 70 | 151.7 | 33.8 | 66.7 | 160.1 | 22.9 | 37 | 153.9 | 24.4 | 24.8 | 37 | 6 | 0.0031 |
| P19035 | Apolipoprotein C-III | 185.1 | 104.9 | 21.7 | 167 | 117.9 | 26 | 64.7 | 90.1 | 7.6 | 45.2 | 46 | 5 | 0.0054 |
| F1N514 | CD5 antigen-like precursor | 122.2 | 184.4 | 19.8 | 102 | 129.6 | 17.7 | 44.3 | 80.6 | 6.8 | 32.1 | 33.4 | 4.4 | 0.0079 |
| A8YXY3 | Selenoprotein F | 119.9 | 117.7 | 11.5 | 133.6 | 105.3 | 13.9 | 95.2 | 125.3 | 6.2 | 119.7 | 101.5 | 11.6 | 0.0089 |
| P60712 | Actin, cytoplasmic 1 | 95.6 | 157.6 | 26.9 | 90 | 128.8 | 14.7 | 59.6 | 121.1 | 10.4 | 57.2 | 60.3 | 7.2 | 0.0105 |
| G5E5T5 | Immunoglobulin M heavy chain secretory form | 117.7 | 174.6 | 19.5 | 104.9 | 129.4 | 18.7 | 47.2 | 87.9 | 7.2 | 45 | 46.6 | 5.2 | 0.0111 |
| A8DC37 | Fc-gamma-RII-D | 133.6 | 175.8 | 20.3 | 175.1 | 120.9 | 25.8 | 64.1 | 105.1 | 10.7 | 46.8 | 42.8 | 4.6 | 0.0111 |
| G5E513 | IgM heavy chain constant region, secretory form, partial | 122.3 | 179.5 | 20.3 | 104.6 | 133.3 | 20.7 | 43.4 | 86.9 | 7.3 | 34 | 38.9 | 4.7 | 0.0113 |
| P79105 | Protein S100-A12 | 81.4 | 151.4 | 22.8 | 82 | 160.8 | 20.7 | 35.3 | 151.3 | 24.7 | 21.4 | 39.2 | 7.1 | 0.0123 |
| P13753 | BOLA class I histocompatibility antigen, alpha chain BL3–7 | 85.2 | 129.9 | 14.1 | 131.4 | 124.2 | 31.6 | 69.6 | 132 | 11.1 | 77 | 73.2 | 9.8 | 0.0142 |
| F1N726 | Glycoprotein 2 | 69.7 | 126.1 | 17.4 | 90 | 174.4 | 19.4 | 38.6 | 150.6 | 16.5 | 39.1 | 63.2 | 10.9 | 0.0156 |
| Q8SQ28 | Serum amyloid A protein | 146.6 | 101 | 16.4 | 137.4 | 129.5 | 32.4 | 81.2 | 137.2 | 16.1 | 54.4 | 80.7 | 13.7 | 0.0185 |
| P01888 | Beta-2-microglobulin | 56.9 | 163.2 | 65.7 | 74.5 | 111.5 | 24.1 | 39.9 | 140.3 | 23.2 | 36.5 | 34.2 | 7.9 | 0.0198 |
| Q32PA1 | CD59 molecule (CD59 blood group) | 134.8 | 109.6 | 9.4 | 117.8 | 107.1 | 15.5 | 85.9 | 130.4 | 13.5 | 100.5 | 123.1 | 20.3 | 0.0256 |
| G3X7A5 | Complement C3 | 96.4 | 150.4 | 24.8 | 91.3 | 90.8 | 12.5 | 68.2 | 136.2 | 17.7 | 75.1 | 67.1 | 9.2 | 0.0343 |
| P17697 | Clusterin | 153.1 | 144 | 15.8 | 171.3 | 108.2 | 19.5 | 61 | 89.7 | 10.5 | 34.8 | 39.3 | 5.4 | 0.0357 |
| F1MLW8 | Immunoglobulin lambda-1 light chain-like isoform X5 | 101.6 | 166.3 | 28.6 | 75.1 | 93.3 | 14.7 | 32.9 | 105.8 | 23.5 | 38.7 | 31.7 | 5.2 | 0.0359 |
| P15497 | Apolipoprotein A-I | 160.1 | 110.2 | 14.5 | 173.4 | 108.5 | 24.2 | 109.3 | 123.4 | 10.6 | 79.4 | 67.9 | 7.6 | 0.039 |
| A0A0A0MP90 | Histone H2A | 58.5 | 125.4 | 37 | 65.4 | 147.1 | 22 | 42.7 | 163.6 | 34.3 | 24.5 | 36.9 | 8.8 | 0.0396 |
| F1N1I6 | Gelsolin | 103.1 | 146 | 18.6 | 95.1 | 120.3 | 12.9 | 75.4 | 115 | 8.7 | 69 | 62 | 8.4 | 0.0426 |
| A0A0N4STN1 | Cathelicidin-1-like | 76.7 | 155.5 | 20.6 | 73.3 | 127.8 | 14.9 | 54.9 | 137.6 | 10.3 | 53.9 | 73.4 | 12.1 | 0.0448 |
| F1MUD2 | Histone H2B | 40.2 | 136.8 | 52.1 | 63.6 | 140.8 | 19.9 | 36.8 | 171.9 | 37.4 | 24.6 | 41.1 | 9.5 | 0.0463 |