Fig. 2.

Effect of HOTAIR knockdown on BC progression. MCF-7 and MDA-MB-231 cells were transfected with si-NC, si-HOTAIR#1 or si-HOTAIR#2. a, b QRT-PCR was performed to test HOTAIR expression to assess the transfection efficiency of si-HOTAIR#1 and si-HOTAIR#2. c, d CCK-8 assay was conducted to assess the effect of HOTAIR knockdown on the proliferation ability of BC cells. e, f Transwell assay was employed to verify the suppression effect of silenced HOTAIR on the migration and invasion of BC cells. g The relative expression level of p-AKT/AKT was detected by WB analysis. *P < 0.05