Table 2.
Expression stability and ranking of reference genes, evaluated based on ΔCt method, BestKeeper, NormFinder, and geNorm.
| Reference gene | ΔCt | BestKeeper | NormFinder | geNorm | Comprehensive ranking | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean SD | Rank | SD | Rank | Coeff. of corr | Rank | General rank | Stability value | Rank | M value | Rank | Geometric mean of ranks | General rank | |
| ACTB | 0.739 | 9 | 0.650 | 9 | 0.777* | 7 | 10 | 0.678 | 13 | 0.591 | 10 | 10.400 | 11 |
| B2M | 1.185 | 14 | 0.697 | 11 | 0.001 | 14 | 14 | 0.891 | 14 | 0.751 | 14 | 14.000 | 14 |
| G6PD | 0.838 | 13 | 0.818 | 13 | 0.816* | 6 | 12 | 0.500 | 12 | 0.674 | 13 | 12.490 | 13 |
| GAPDH | 0.654 | 5 | 0.337 | 3 | 0.610* | 11 | 7 | 0.168 | 6 | 0.404 | 4 | 5.384 | 5 |
| GUSB | 0.651 | 4 | 0.110 | 1 | 0.596* | 12 | 2 | 0.127 | 3 | 0.386 | 3 | 2.913 | 4 |
| HMBS | 0.788 | 11 | 0.827 | 14 | 0.876* | 4 | 9 | 0.162 | 5 | 0.620 | 11 | 8.590 | 9 |
| HPRT1 | 0.805 | 12 | 0.635 | 8 | 0.642* | 10 | 13 | 0.290 | 9 | 0.647 | 12 | 11.393 | 12 |
| PGK1 | 0.699 | 7 | 0.762 | 12 | 0.930* | 2 | 5 | 0.199 | 7 | 0.476 | 6 | 6.192 | 6 |
| RPL13A | 0.698 | 6 | 0.544 | 6 | 0.751* | 8 | 8 | 0.225 | 8 | 0.535 | 8 | 7.445 | 7 |
| RPLP0 | 0.568 | 2 | 0.342 | 4 | 0.869* | 5 | 4 | 0.128 | 4 | 0.339 | 1 | 2.378 | 3 |
| RPS18 | 0.746 | 10 | 0.307 | 2 | 0.106 | 13 | 6 | 0.323 | 10 | 0.512 | 7 | 8.050 | 8 |
| TBP | 0.560 | 1 | 0.416 | 5 | 0.906* | 3 | 3 | 0.068 | 1 | 0.339 | 1 | 1.316 | 1 |
| TFRC | 0.634 | 3 | 0.651 | 10 | 0.936* | 1 | 1 | 0.068 | 1 | 0.443 | 5 | 1.968 | 2 |
| YWHAZ | 0.718 | 8 | 0.567 | 7 | 0.721* | 9 | 10 | 0.440 | 11 | 0.564 | 9 | 9.434 | 10 |
*P < 0.05.