Table 3.
Primers of target genes.
| Assay | Symbol | Name | Function | Assay ID |
|---|---|---|---|---|
|
PrimePCR SYBR Green Assay (Bio-Rad) |
TBP | TATA box binding protein | Transcription factor binding | qHsaCID0007122 |
| POU5F1 | POU class 5 homeobox 1 | Marker for undifferentiated cells | qHsaCED0038334 | |
| NANOG | Nanog homeobox | Marker for undifferentiated cells | qHsaCED0043394 | |
| ALP | Alkaline phosphatase, liver/bone/kidney | Matrix mineralization | qHsaCED0045991 | |
| OC | Osteocalcin | Calcium-binding protein hormone | qHsaCED0038437 | |
| DMP1 | Dentin matrix acidic phosphoprotein 1 | Extracellular matrix mineralization | qHsaCID0015301 | |
| SOST | Sclerostin | Produced primarily by the osteocyte | qHsaCED0046506 | |
|
Taqman Gene Expression Assay (Applied Biosystems) |
RUNX2 | Runt related transcription factor 2 | Key transcription factor for osteoblast differentiation | Hs00231692_m1 |
| SP7 | Sp7 transcription factor | Transcription factor for osteoblast differentiation | Hs01866874_s1 | |
| COL1A1 | Collagen type I alpha 1 | Fibrillar collagen | Hs00164004_m1 |