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. 2020 Jul 16;3:385. doi: 10.1038/s42003-020-1104-0

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© The Author(s) 2020, corrected publication 2022

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Fig. 2 — a, b KCNQ1 currents recorded from a Xenopus oocyte co-expressed with CiVSP in response to voltage pulses to +60 mV (the voltage protocol is depicted in the inset in (a). Currents of the first trace control (black), after rundown (gray), and after injection of ~10 µM CP1 into oocytes (red) are shown (a). Averaged time course of normalized current amplitude of KCNQ1 with rundown (black) and after CP1 injection (red) (b) (n = 3). c, d I_Ks (KCNQ1 + KCNE1) co-expressed with CiVSP in response to voltage pulses to +60 mV. Currents of the first trace control (black), after rundown (gray), after bath application of 10 µM CP1 (red), and after bath application of 100 µM chromanol 293B (blue) are shown (c). Averaged time course of normalized current amplitude of I_Ks with rundown (black), CP1 application (red), and chromanol 293B (d) (n = 7). e, f I_Ks currents recorded in the inside-out patch in response to voltage pulses to +80 mV (the voltage protocol is depicted in the inset in (e). Representative I_Ks current traces ran down after patch excision due to PIP₂ depletion (e, upper), and rescued by 10 µM CP1 application (e, lower). The changing color of the current traces and arrows indicates the time sequence of rundown and rescue (e). Normalized current amplitude following patch excision and CP1 application (n = 3) (f).