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. 2020 Jul 16;10:11715. doi: 10.1038/s41598-020-68638-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Box plot histograms showing the degree of colocalization between immunoreactivity for NKCC1 and selected axonal and glial markers in laminae I–II of the spinal dorsal horn. (a) Percentage of profiles immunoreactive for the applied axonal and glial markers that were also labeled for NKCC1. (b) Percentage of profiles immunoreactive for NKCC1 that were found within the confines of areas immunostained for the applied axonal and glial markers.