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. 2020 Jul 17;15:47. doi: 10.1186/s13027-020-00312-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Hematoxylin–Eosin staining in cervical tissue sample (× 200). a, normal cervical tissue. The cervical tissue were mainly composed of stromal tissue (fibrous connective tissue), blood vessels, and non-keratinized stratified squamous epithelium. b, Cervical tissue samples from women with CIN I. The nuclei of the dysplastic cells were enlarged, irregular, and hyperchromatic, and the histopathologic evaluation of these patients demonstrated a slight dysplasia limited to the basal third of the cervical epithelial layer. c and d, Cervical tissue samples from women with CIN II and III. Moderate or severe dysplasia and more abnormal cells were detected in at least two-thirds of the epithelial layer of the cervical samples in CIN II or III patients. There was an increased nuclear-to-cytoplasmic ratio, loss of cell polarization, and mitotic figures present in dysplastic cells of CIN II-III patients. e, epithelium; s, stroma; bv, blood vessels; arrow, mononuclear cells