Fig. 3.

Apoptosis efficiency induced by ADI△(C398A) expressed in MRC5, HepG2 and PC3 cells. Cells were separately transfected by pcDNA4, pcDNA4-ADI△ and pCMV-FTL plasmids. Cell apoptosis rates were detected by flow cytometry after the static cell culture for 72 h. a: Representative images of FACS analysis of annexin V and PI staining of MRC5, HepG2 and PC3 cells. b: Death ratio summary of FACS analysis from Fig. 3a. c: Fluorescence assay for mitochondrial permeability transition pore (MPTP) from Fig. 3a. d: Immunoblots of ADI△ and ASS expression in MRC5, HepG2 and PC3 cells. C-myc-tag antibody was used to detect c-myc-tag-fused ADI△. FLAG tag was used to detect overexpressed FTL. The blot of GAPDH was from the same gel as the blot of FTL. Full-length blots are presented in Supplementary Fig. S3