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. 2020 Jul 16;20:665. doi: 10.1186/s12885-020-07133-4

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Molecular mechanism of cell apoptosis induced by arginine deprivation. a: mRNA level detection of some factors related with mitochondria apoptosis pathway by Quantitative RT-PCR in PC3 and HepG2 cells. b: Immunoblot of the factors related with apoptosis pathway in PC3 and HepG2 cells. Full-length blots are presented in Supplementary Fig. S4. c: Fluorescence assay for mitochondrial permeability transition pore (MPTP). d: Activity assay of Caspase 3 through caspase 3 assay kit (Colorimetric) (abcam. ab39401). e: Activity assay of Caspase 9 through caspase 9 assay kit (Colorimetric) (abcam. Ab65608). f/g: The relative quantification for protein expressions in PC3 and HepG2 cell lines. Grey scales of protein bands from Fig. 4b were detected by ImageJ 1.52. P values were calculated by comparing pcDNA4-ADI plasmids-treated cells with pcDNA4 plasmid-treated cells in the respective cell lines. **P < 0.01; ***P < 0.001