Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 16;10:11807. doi: 10.1038/s41598-020-68713-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Comparative analysis for the expression and phosphorylation of GSK3β in human ESCC cells (TE-1, TE-5, TE-8, TE-9, TE-10, TE-15, KES), normal esophageal squamous epithelial cells (TYNEK-3), and normal squamous mucosa and primary tumors from ESCC patients. (A) Expression of GSK3β and of its phosphorylated forms (pGSK3β^S9, inactive form; pGSK3β^Y216, active form) were examined by Western blotting. β-actin expression was monitored as a loading control in each sample. (B) Representative findings for the expression of GSK3β and its Y216 phosphorylated fraction (pGSK3β^Y216) in the primary tumor and corresponding normal squamous mucosa of ESCC patients. The scale bar indicates 100 μm in length. Immunohistochemical images were captured using Keyence BZ-X700 Analyzer (Version 1.3). The two right hand graphs generated using GraphPad Prism 5.0 (GraphPad Software, Inc. CA) show statistical comparison of the immunohistochemistry (IHC) scores for GSK3β and pGSK3β^Y216 between the primary tumor (T) and normal mucosa (N) of ESCC patients. A horizontal bar in each group shows the mean value of IHC scores. (C) Expression of GSK3β mRNA in normal esophageal tissues (N) and primary ESCC tumor tissues (T) based on the TCGA database. The data was generated using the analysis tool UALCAN (https://ualcan.path.uab.edu/)³³. n, number of patients; **P < 0.01. Full-length blots for (A) are shown in Supplementary Information, Fig. S9.