Table 3.
Mitochondrial CNV of STRs within a D. silvarum individual
| Position | Gene | Ref | Allele | Depth |
|---|---|---|---|---|
| 1810 | COI | [G]8 | Ref/+ 1G/−1G/+2GG/T/G/C/+ 1 T/*/−2GG | 352,526/6177/3116/150/32/25/19/19/10/6 |
| 2573 | COI | [A]9 | Ref/+ 1A/− 1A/T/+2AA/A/+3AAA/−2AA | 277,857/7636/1788/223/170/13/9/8 |
| 3441 | tRNA-Asp | [A]8 | Ref/+ 1A/+2AA/−1A/+ 1C/+3AAA/+ 1G/G/C/A | 438,644/195894/3402/1011/69/58/17/10/7/5 |
| 3619 | ATP8/6 | [A]10 | Ref/+ 1A/−1A/+2AA/−2AA/T/+3AAA/A/C | 400,525/23182/9717/997/185/137/49/14/11 |
| 4592 | COIII | [T]11 | Ref/+ 1 T/−1 T/+2TT/−2TT/+3TTT/T/+ 1A | 107,531/10859/6363/1027/140/110/20/10 |
| 5524 | tRNA-Ala | [A]10 | Ref/+1A/−1A/+2AA/−2AA/A/+3AAA | 97,659/3329/2622/131/27/19/9 |
| 6361 | ND1 | [A]10 | Ref/+1A/−1A/+2AA/−2AA/+3AAA/C | 94,957/4984/3410/313/18/17/8 |
| 7324 | 16S rRNA | [TA]9 | Ref/−2TA/+2TA | 93,454/4152/3088 |
| 7737 | 16S rRNA | [A]9 | Ref/+1A/−1A/+2AA/C/A | 89,380/1277/890/32/6/5 |
| 8042 | 16S rRNA | [T]9 | Ref/+1 T/−1 T/+2TT/−2TT/G | 96,225/1795/1552/40/8/5 |
| 8243 | 12S/tRNA-Val | [T]10 | Ref/+1 T/−1 T/+2TT/−2TT/+3TTT/G | 99,234/3993/2326/151/31/8/7 |
| 8885 | 12S rRNA | [T]10 | Ref/+1 T/−1 T/+2TT/−2TT/T/+3TTT | 86,875/4767/3630/287/38/36/18 |
| 10,026 | ND5 | [A]10 | Ref/−1A/+1A/+2AA/−2AA/+3AAA/C | 99,166/5465/3630/134/82/6/5 |
| 10,298 | ND5 | [A]13 | Ref/−1A/+1A/−2AA/+2AA/−3AAA/+3AAA/C | 91,464/17434/7221/2402/721/241/61/5 |
| 11,216 | ND5 | [A]17 | Ref/−1A/+1A/−2AA/+2AA/−3AAA/+3AAA/A/*/+2TA/+ 1C | 138,074/26622/18595/4443/2859/542/413/96/24/5/5 |
| 11,483 | ND4/4 L | [TA]6 | Ref/−2TA/+2TA/A/T | 200,829/3596/2031/19/7 |
| 11,962 | ND4/4 L | [A]11 | Ref/−1A/+1A/−2AA/+2AA/C/+3AAA/A/−3AAA | 203,481/18678/10550/646/496/28/20/11/10 |
| 12,082 | ND4/4 L | [A]8 | Ref/−1A/+1A/T/+2AA/A | 215,342/1501/821/48/12/7 |
| 12,505 | ND4/4 L | [A]9 | Ref/−1A/+1A/+2AA/C/−2AA | 227,869/3067/2631/47/20/9 |
| 12,697 | ND4/4 L | [A]8 | Ref/+1A/−1A/+2AA/C/A/G | 230,254/1922/497/23/22/6/6 |
This reference sequence (GenBank: MN347015) uses the consensus DNA sequence of one Dermacentor silvarum individual collected from Qiangyang, Gansu province of China. All the STR variants were selected using a very strict parameter (Methods). Ref was designated as the STR reference, as it occured the most frequently in mtDNAs within one individual tick. In the allele column, insertions and deletions were noted as “+” and “-” following the specifications in pileup format [20]. The numbers in the depth column are one-to-one corresponding to the variants in the allele column. For example, the STR position 1810 had a reference sequence [G]8 and a variant [G]9 noted by “+G”, which had a depth 6177