Table 1.
Microinjection results of bZip-mNG, bZip-tTA and bZip-tTA-mNG constructs
| Construct | No. of embryos injected | Larvae hatching (%)a | Survival (%) | No. of screened larvaeb | No. of DsRed positive larvae | No. of transgenic lines | Transformation rate (%)c |
|---|---|---|---|---|---|---|---|
| bZip-mNG | 2126 | 424 (20) | 415 (19) | 20,484 | 104 | 3 | 1.4 |
| bZip-tTA | 1825 | 634 (35) | 634 (35) | 30,365 | 14 | 3 | 0.9 |
| bZip-tTA-mNG | 2065 | 399 (19) | 370 (18) | 50,518 | 50 | 1 | 0.5 |
aPercentage of injected embryos that hatched into L3-L4 larvae
bPercentage of injected embryos that hatched into adults
cPercentage of independent transformed lines generated per fertile adult (assumes ~ 50% fertility)