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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Mol Microbiol. 2020 Apr 8;114(1):109–126. doi: 10.1111/mmi.14499

Figure 2.

Figure 2.

Stability of GFP::SpxA1tail and GFP::SpxA2tail fusion proteins in S. mutans. (A) Graphical representation of GFP fused to the last 10 C-terminal amino acids of either SpxA1Smu or SpxA2Smu. (B) Fluorescence decay of GFP::SpxA1tail and GFP::SpxA2tail expressed in UA159 (parent) or ΔclpP strains after addition of chloramphenicol. Asterisks indicate time points showing statistically significant differences (p ≤ 0.01, one-way ANOVA) in decay of GFP expression in the SpxA2tail construct when hosted in UA159 compared to the ΔclpP strain. (C) Western blot analysis of UA159 or ΔclpP expressing GFP::SpxA1tail and GFP::SpxA2tail probed with anti-GFP polyclonal antibody. The images shown are representative of 3 or more independent experiments.