Table 3. HBV and HDV diversity panel results.
| Specimen ID | Country | Virus | Genotype | Final length (nt) | Boot strap | VL (log IU/mL) | Library Type | Total Reads | Viral reads | Reads per million | Percent Genome Coverage | Avg coverage depth |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 23–23 | Cameroon | HBV | E | 440 | 99 | 1.66 | mNGS | 3,963,160 | 594 | 150 | 3% | 1 |
| - | - | - | - | - | - | - | xGen | 2,284,203 | 95 | 42 | 14% | 16 |
| - | - | HDV | 6 | 1685 | 100 | 4.89 | mNGS | 4,583,420 | 73,543 | 16045 | 100% | 4863 |
| - | - | - | - | - | - | - | xGen | 2,322,206 | 2,270,392 | 977688 | 100% | 137586 |
| U160953A | Cameroon | HBV | B2 | 2731 | 98 | 3.24 | mNGS | 1,624,386 | 10 | 6 | 8% | 1 |
| - | - | - | - | - | - | - | xGen | 3,160,577 | 191 | 60 | 84% | 3 |
| - | - | HDV | 1 | 1554 | 100 | 4.59 | mNGS | 1,624,386 | 3,074 | 1892 | 90% | 212 |
| - | - | - | - | - | - | - | xGen | 3,205,212 | 2,926,340 | 912994 | 94% | 204100 |
| - | - | - | - | - | - | - | Pan viral | 1,162,542 | 390,959 | 336297 | 90% | 369999 |
| U22343 | Cameroon | HBV | A3 | 3221 | 100 | nd | mNGS | 1,583,088 | 859 | 543 | 100% | 19 |
| - | - | - | - | - | - | - | xGen | 3,548,047 | 879,717 | 247944 | 100% | 19624 |
| - | - | HDV | 8 | 1676 | 100 | 6.98 | mNGS | 1,904,026 | 2,136 | 1122 | 100% | 119 |
| - | - | - | - | - | - | - | xGen | 3,628,496 | 2,666,770 | 734952 | 100% | 169534 |
| 0111A838 | USA | HBV | B2 | 3215 | 100 | 5.92 | mNGS | 1,531,143 | 173 | 113 | 92% | 3 |
| - | - | - | - | - | - | - | xGen | 55,902 | 44,229 | 791188 | 100% | 923 |
| - | - | - | - | - | - | - | Pan viral | 690,304 | 40,003 | 57950 | 100% | 1768 |
| 1010A324 | USA | HBV | C1 | 2814 | 100 | 4.18 | mNGS | 1,354,611 | 14 | 10 | 3% | 1 |
| - | - | - | - | - | - | - | xGen | 9,880 | 1,034 | 104656 | 87% | 26 |
| - | - | - | - | - | - | - | Pan viral | 405,484 | 321 | 792 | 78% | 18 |
| 1007-HBV-0036 | Peru | HBV | F1 | 2901 | 100 | 3.6 | mNGS | 2,781,836 | 282 | 101 | 6% | 1 |
| - | - | - | - | - | - | - | xGen | 12,905 | 931 | 72143 | 90% | 27 |
| - | - | - | - | - | - | - | Pan viral | 401,432 | 255 | 635 | 63% | 18 |
| 1019-HBV-0001 | Republic of Congo | HBV | E | 2479 | 100 | 3.53 | mNGS | 1,554,926 | 123 | 79 | 15% | 1 |
| - | - | - | - | - | - | - | xGen | 54,413 | 6,495 | 119365 | 76% | 193 |
| Pan viral | 22,393,622 | 4,162 | 186 | 70% | 264 | |||||||
| 0211A172 | USA | HBV | H | 3178 | 100 | 3.52 | mNGS | 478,506 | 35 | 73 | 21% | 1 |
| - | - | - | - | - | - | - | xGen | 16,763 | 9,313 | 555569 | 98% | 215 |
| - | - | - | - | - | - | - | Pan viral | 147,056 | 1,746 | 11873 | 81% | 96 |
| 0610A006 | Egypt | HBV | D1 | 1496 | 91 | 3.48 | mNGS | 2,582,798 | 11 | 4 | 6% | 2 |
| - | - | - | - | - | - | - | xGen | 56,962 | 406 | 7128 | 47% | 12 |
| - | - | - | - | - | - | - | Pan viral | 424,558 | 291 | 685 | 58% | 22 |
| U160953A | Cameroon | HBV | B2 | 2731 | 98 | 3.24 | mNGS | 1,624,386 | 10 | 6 | 8% | 1 |
| - | - | - | - | - | - | - | xGen | 3,160,577 | 191 | 60 | 84% | 3 |
| - | - | - | - | - | - | - | Pan viral | 1,162,542 | 0 | 0 | 0% | 0 |
| HBV0073 | Spain | HBV | D | 56 | nd | 1.34 | mNGS | 76,111 | 22 | 289 | 1% | 2 |
| - | - | - | - | - | - | - | xGen | 2,044 | 4 | 1957 | 10% | 1 |
| - | - | - | - | - | - | - | Pan viral | 21,630 | 0 | 0 | 0% | 0 |
| HBV0285 | Spain | HBV | A2 | 43 | nd | <1.18 | mNGS | 1,969,686 | 6 | 3 | 6% | 1 |
| - | - | - | - | - | - | - | xGen | 22,230 | 36 | 1619 | 1% | 29 |
| - | - | - | - | - | - | - | Pan viral | 237,712 | 0 | 0 | 0% | 0 |
Summary of results for HBV and HDV sequencing (mNGS, xGen, and Pan viral) for diversity panel specimens.