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. 2020 Jul 17;15(7):e0236195. doi: 10.1371/journal.pone.0236195

Fig 1. Viral load and Heatr9 expression during acute influenza virus infection in C57BL/6J mice.

Fig 1

Pooled experimental data of Heatr9 expression was tracked over the course of acute influenza virus infection from RNA isolated from PR8 influenza virus infected C57BL/6 mouse lungs (A). The expression of Heatr9 from individual experiments (determined by fold induction compared to uninfected control mouse lungs) was correlated with viral titers at days 3 (B), 6 (C), and 10 (D) post infection. Each dot represents a single mouse. Results representative of 2 or more independent experiments. Viral Units were determined by qRT-PCR based method in which the MP viral transcript was detected from cDNA synthesized from isolated mouse lung RNA. For statistical analysis, (A) a nonparametric One-way ANOVA was performed (Kruskal-Wallis test) with a post hoc Dunn’s multiple comparison test to determine significance (* indicates p<0.05). For correlations (B, C, and D), a Spearman correlation test for significance was performed. Significant correlations are considered those with p<0.05. Each dot represents an individual mouse. Due to insufficient material, the correlation between Heatr9 induction and viral burden could not be performed for all mice. This is the reason why more data points are present in 1A compared to 1D.