Skip to main content
. 2020 Jul 17;15(7):e0236195. doi: 10.1371/journal.pone.0236195

Fig 2. Heatr9 is induced upon viral infection and treatment with cytokines.

Fig 2

A549 human lung cells were infected with 1 MOI of PR8 influenza virus and incubated for 24 hours before RNA was isolated to assay for Heatr9 expression by qRT-PCR (A). A549 cells were infected with RSV-A2 and RNA was isolated at 48 hours post infection. Heatr9 induction was plotted against Isg15 induction (B). A549 cells were infected with 1 MOI of PR8 and incubated for 24 hours prior to removal of culture supernatant, which was then incubated with uninfected A549 cells. RNA was isolated from uninfected A549 incubated with supernatant of infected A549 cells for 24 hours prior to RNA isolation for assaying for Heatr9 induction (C). A549 cells were incubated with cytokine(s) for 16–24 hours in the absence of infection and Heatr9 expression induction was assayed by qRT-PCR (D). For statistical analysis, unpaired Student’s t test (A, C), linear regression analysis (B), and One-way ANOVA with a post hoc Tukey multiple comparisons (D) were performed. Asterisk (*) indicates a p<0.05.