FIGURE 2.

Nc886 directly targets TAP1. A, Westernblot analysis of TAP1, HLA‐A and β‐actin in scramble and nc886⁺ cells. The gray scale ratio (normalized to β‐actin) was calculated by ImageJ software (left). TAP1 5’UTR and HLA‐A 3’UTR plasmids were constructed and transfected into scramble cells or nc886⁺ cells. Dual‐luciferase reporter assays were used to detect direct interactions with nc886 (right). B, The RIP experiment validated the interaction between nc886 and TAP1 or HLA‐A in nc886⁺ cell lines, and the left picture verified that the magnetic beads were successfully loaded with antibodies. C, Schematic diagram and problem of nc886 changing tumor antigen.