FIGURE 4.

Macrophages with engulfed tumor cells accumulate in inflammatory regions of the spleen. A, Transmission electron microscope images of representative macrophages from spleen tissues (n = 3). Scale bar, 10 μm and 2 μm. Triangles indicate enlarged macrophages. B, Representative immunohistochemical images for CD68 and PSA staining in mouse spleen samples (40×) (n = 6). The triangles highlight macrophages in inflammatory regions. The rectangles indicate typical macrophages with PSA or CD68 staining. PBS was used as a negative control. Scale bar, 20 μm. Mouse samples at 28 days are shown in this panel