FIGURE 7.

TGF‐β1 is vital in the process of M2 polarization. A, CCK8 assays were adopted to examine the growth of BM‐derived and splenic (SP) macrophages (left), and of RAW264.7 cells cultured in supernatant from scramble cells or nc886⁺ cells respectively (right). Scramble cells overexpressed TGF‐β1 fivefold more than mimic cells by QRT‐PCR (middle). B, The growth of primary macrophages or RAW264.7 cells upon TGF‐β1 (10 ng/mL) stimulation. C, TGF‐β1 affected the mRNA levels of CD206, CD163, IL‐6, IL‐10, and TNF‐α in RAW264.7 cells. At least three independent experiments are shown as the mean ± SD