FIGURE 5.

Gene expression changes induced by BI‐D1870 in MCL‐derived cell lines. A,B. KPUM‐YY1 and Jeko‐1 cells were treated with IC₈₀ concentrations of BI‐D1870 for 6 h. Correlation coefficients of commonly upregulated (>2‐fold) and downregulated (<0.5‐fold) genes between Jeko‐1 and KPUM‐YY1 cells were 0.63 and 0.27, respectively (A). B, Mean expression levels of 542 commonly downregulated genes Jeko‐1 and KPUM‐YY1 cells after BI‐D1870 treatment relative to untreated cells, identified by microarray analysis. Several genes involved in lymphomagenesis, B‐cell receptor signaling, and B cell development are listed. C, Relative expression levels of c‐MYC, MYB, BCL2, BCL2L1, BLNK, CD79B, PAX5, IKZF1, and TNFRSF17 determined by quantitative RT‐PCR in two cell lines treated with BI‐D1870 at their respective IC₈₀s for 6 h. Expression levels were defined as 1.0 in untreated cells. Data are shown as the mean ± SD of three independent experiments. D, Gene set enrichment plots for representative target gene sets of MYC significantly enriched in Jeko‐1 cells treated by BI‐D1870 (BID) (left) and of TNF‐α signaling via NF‐kB pathway significantly enriched in KPUM‐YY1 cells treated by BID (right) compared with untreated cells (Ctrl)