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. 2020 Jul 14;11(28):2747–2762. doi: 10.18632/oncotarget.27668

Figure 4. TMEM165 knockout inhibits breast cancer growth in a xenograft model.

Figure 4

(A) Representative image of tumors extracted from nude mice treated with MDAMB231 Control and MDA MB231 TMEM165KO. (B) Cumulative growth curves for MDAMB231 control cells and MDAMB231 TMEM165KO cell xenografts (n = 10 mice control, n = 10 TMEM165KO; * P < 0.0001). (C) Representative images of MDAMB231 Control and TMEM165KO derived tumor sections stained for Hematoxylin and eosin (H&E), Ki67, Vimentin, E-Cadherin, and CD31, bars 100 μm. (D) Western blot analysis of vimentin and E-cadherin expression after transient transfection with TMEM165/GFP or vector in TMEM165KO and control cells, asterisks mark the vimentin band in Western blots. Lower panels shows the quantification of Vimentin and E-cadherin protein levels after normalization with ERK (n = 2, ** P < 0.01). The Western blot images are cropped and were imaged with Bio-Rad EZ Imager. The second band from the left on the ERK Western blot has a slight air bubble just above the ERK band that does not impair quantitation.