Video 2. Conformational changes of a hydrophilic arm of the a-subunit linked to movement of the EG subunits.

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A morphed movie focusing on the conformational changes of a hydrophilic arm of the a-subunit between isolated V_o and V_o in the holo-enzyme. EG_p and EG_d indicate proximal and distal EG subunits. The position of EG_d was determined from the low-resolution density map (Figure 6—figure supplement 1). The hydrophilic arm of the a-subunit associated with binding EG_d to one of the B subunits from V₁, is forced to swing away from the d-subunit, resulting in disruption of the specific interaction between the subunits.