Table 1. CryoEM data collection, refinement and model statistics.
| TthV/A-ATPase | V1EGdasol | Isolated Vo | |
|---|---|---|---|
| Data collection | |||
| Electron microscope | Titan Krios | CRYOARM200 | |
| Electron detector | Falcon II | K2 summit | |
| Magnification | 75,000 | 50,000 | |
| Voltage (kV) | 300 | 200 | |
| Electron exposure (e-/Å2) | 91 | 79.2 | |
| Defocus range (μm) | 2.4–3.0 | 1.0–3.0 | |
| Pixel size (Å) | 1.1 | 1.1 | |
| Movie No. | 3694 | 5988 | |
| Frame per movie | 34 | 60 | |
| Automation software | EPU | JADAS | |
| Data processing | |||
| Total extracted particles | 3.5 × 105 | 3.14 × 106 | |
| Total particle after 2D | 144,758 | 706,617 | |
| Resolution (Å) | 3.6 | 3.5 | 3,93 |
| Sharpening B-factor | −81.07 | −60.25 | −110.87 |
| EMDB ID | 30013 | 30014 | 30015 |
| Model building and refinement | |||
| Initial models | - | 5Y5Y | 5Y5X, 1V9M |
| Building and refinement package | - | COOT, phenix | COOT, phenix |
| Total atom No. | - | 26,631 | 13,888 |
| Total residue No. | - | 3418 | 1894 |
| Total chain No. | - | 8 | 16 |
| Ligands | - | ADP | - |
| cc_mask | - | 0.85 | 0.82 |
| Ramachandran favored | - | 88.89% | 92.75% |
| Ramachandran outliers | - | 0.03% | 0.00% |
| Rotamer outliers | - | 11.45% | 0.37% |
| c-beta deviation | - | 0 | 0 |
| CaBLAM outliers | - | 5.94% | 2.84% |
| Clashscore | - | 6.38 | 20.01 |
| RMSD bonds (Å) | - | 0.006 | 0.006 |
| RMS angle (°) | - | 0.669 | 0.725 |
| MolProbity score | - | 2.73 | 2.26 |
| PDB ID | - | 6LY8 | 6LY9 |