Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | Rosetta (DE3) pLys competent cells | Novagen | Cat#71403 | |
| Strain, strain background (Escherichia coli) | Rosetta (DE3) competent cells | Millipore Sigma | Cat#70954 | |
| Biological sample (Bos taurus) |
Bovine brain tubulin | Lab purification | Protocol adopted from Castoldi and Popov, 2003 | |
| Antibody | Penta-HIS biotin conjugate, monoclonal mouse | Qiagen | Cat#34440 | |
| Chemical compound, drug | Glucose oxidase | Millipore Sigma | Cat#345386 | |
| Chemical compound, drug | Catalase | Millipore Sigma | Cat#219261 | |
| Chemical compound, drug | Biotinylated bovine serum albumin (BSA) | Vector laboratories | Cat#B-2007 | |
| Chemical compound, drug | Avidin DN | Vector laboratories | Cat#A-3100 | |
| Chemical compound, drug | TCEP | Thermo Fischer | Cat#T2556 | |
| Software, algorithm | Labview | National Instruments | RRID:SCR_014325 | |
| Software, algorithm | Igor Pro | Wavemetrics | RRID:SCR_000325 | |
| Software, algorithm | R | R Foundation for Statistical Computing |