Table 2. Plasmids used in this study.
| Protein complex | Plasmid name | Names used in this paper | Proteins expressed* | Vector | References |
|---|---|---|---|---|---|
| Mif2 | Sc_Mf_7 | Mif2 | Mif2-linker-(27-392)MBP-6XHis** | pLIC | This study |
| pGH52 | Mif2 | Mif2-linker-(27-392)MBP** | pLIC | This study | |
| Sc_Mf_5B | ΔN-Mif2 | (41-549)Mif2-linker-(27-392)MBP | pLIC | This study | |
| OA | pGH3 | OA | Ame1-6XHis, Okp1 | pST39 | This study |
| pGH4 | OA | Ame1-FLAG, Okp1 | pST39 | This study | |
| pGH42 | ΔN-OA | (21-324)Ame1-FLAG, Okp1 | pST39 | This study | |
| pGH15 | ΔN-OA | (21-324)Ame1-6XHis, Okp1 | pST39 | This study | |
| MIND | pGH63 | 2D-MIND | 6XHis-linker-Nsl1, S240D, S250D-Dsn1, Mtw1, Nnf1 | pST39 | This study |
| pGH62 | 2D-MIND | FLAG-Nsl1, S240D, S250D-Dsn1, Mtw1, Nnf1 | pST39 | This study | |
| pGH46 | MIND | Nsl1, FLAG-Dsn1, Mtw1, Nnf1 | pST39 | This study | |
| Ndc80c | pJT048 | Part of Ndc80c | Spc24-Flag, Spc25 | pRSFDuet | Kudalkar et al., 2015 |
| pEM033 | Part of Ndc80c | Spc24-6XHis, Spc25 | pRSFDuet | Scarborough et al., 2019 | |
| Ndc80/Nuf2 | Part of Ndc80c | Nuf2, Ndc80 | pETDuet | Wei et al., 2005 | |
| Dam1c | pJT044 | Dam1c | Dad1, Duo1, Spc34-FLAG, Dam1, Hsk3 and Dad4, Dad3, Dad2, Spc19, Ask1‡ |
pST39 | Umbreit et al., 2014 |
| CI | pGH58 | CI | FLAG-Chl4, Iml3 | pLIC | This study |
| Histones | pScKl2 | Cse4-NCP | K.lactis 6XHis-H2A, K. lactis 6XHis-H2B, Cse4, K. lactis 6XHis-H4 | pLIC | Migl et al., 2020 |
| pScKl4 | H3-NCP | H3, 6XHis-H2A, H2B. K.lactis 6XHis-H4 | pLIC | Migl et al., 2020 | |
| pScHT4 | Cse4(1-50) | 6XHis-MBP-(1-50)Cse4 | pLIC | This study |