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. 2020 Jul 17;10:11913. doi: 10.1038/s41598-020-68639-2

Figure 2.

Figure 2

Compression alters peritoneal mesothelial morphology and surface ultrastructure in vitro and ex vivo. (A) LP9 human peritoneal mesothelial cells or primary human mesothelial cells (HPMC) were cultured in control or compressed conditions using a Flexcell Compression Plus System (~ 3 kPa; ~ 22 mmHg) for 24 h. Cells were fixed with 4% PFA buffer and stained with Phalloidin488 and DAPI. Cells were imaged with Leica DM5500 fluorescence microscope at × 20 magnification. Scale bar, 200, 130 µm. (B) Murine peritoneal explants were maintained ex vivo under control or compressed conditions (~ 3 kPa; ~ 22 mmHg) for 1 h. Explants were fixed and processed for imaging by FEI-Magellan 400 Field Emission Scanning Electron Microscope. Scale bar, 10, 5, 4, 2 µm (C) Tunneling nanotubes (TNT) were quantified using ImageJ. Three mice were included in each group with 10 images analyzed for each mouse. All results are presented as mean ± s.e.m. and P-values were calculated using a Student’s two-tailed t-test. Triple asterisk indicates significant p-value < 0.01.