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. 2020 Jul 17;10:11913. doi: 10.1038/s41598-020-68639-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Compression-induced nanotubes adopt distinct morphologies and participate in mitochondria transport between LP9 mesothelial cells and OvCa cells. (A) High magnification scanning electron micrographs of TNT formed under compression (~ 3 kPa; ~ 22 mmHg) in murine peritoneal explants. The peritoneal explants were compressed ex vivo for 1 h, fixed and processed for imaging using a FEI-Magellan 400 Field Emission Scanning Electron Microscope. Yellow arrows refer to distensions in the nanotubes. (B) GFP-tagged LP9 human peritoneal mesothelial cells were incubated with MitoTracker red to label mitochondria, then co-cultured with OVCAR5 or OVCAR8 cells under compression (~ 3 kPa; ~ 22 mmHg) for 24 h and stained with DAPI. Cells were imaged with Leica DM5500 fluorescence microscope at × 20 magnification. Top panel: phase image showing LP9 and OVCAR cells; second panel: GFP-tagged LP9 peritoneal MC; third panel: MitoTracker red showing labeled mitochondria in LP9 cells and extracellular projections; fourth panel: DAPI-stained nuclei of both LP9 and OVCAR cells; fifth panel: overlay. Yellow arrows denote nanotubes or labeled mitochondria transferred in nanotubes.