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. 2020 May 18;14(8):2179–2196. doi: 10.1038/s41396-020-0679-2

Fig. 2. The effect of non-antibiotic pharmaceuticals causing increased ROS generation and altering the cell membrane integrity in A. baylyi.

Fig. 2

a Fold changes of ROS fluorescence intensity under the exposure of non-antibiotic pharmaceuticals. b Fold changes of ROS fluorescence intensity with the addition of the ROS scavenger thiourea (5 mg/L of non-antibiotic pharmaceuticals dosage). c Fold changes of transformation frequency with the addition of ROS scavenger thiourea and 5 mg/L of non-antibiotic pharmaceuticals. d Fold changes of fluorescence intensity on PI-stained cells under the exposure of non-antibiotic pharmaceuticals. Significant differences between non-antibiotic-dosed samples and the control were analysed by independent-sample t-test and corrected by Bonferroni correction method, *P* < 0.05, **P* < 0.01 and ***P* < 0.001. For figures b and c, significant differences between the groups with and without thiourea dosage were analysed by independent-sample t-test, *P < 0.05, **P < 0.01 and ***P < 0.001.