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. 2020 Jul 17;11(7):544. doi: 10.1038/s41419-020-2731-6

Fig. 2. Vimentin and E-cadherin expression levels in proximal tubular cells of kidneys in Control, ZnT8-KO, db/db and ZnT8-KO-db/db mice.

Fig. 2

In the next experiments, immunofluorescence staining implicated the localization of Vimentin (red) and E-cadherin (red) in the proximal tubular cells of kidneys of Control (a1-3), ZnT8-KO mice (b1-3), db/db (c1-3) and ZnT8-KO-db/db mice (d1-3) and data are represented in a, b, respectively. AQP1 (green) is the marker for tubular cells. Original magnification, ×400. Data from Western blotting analyses in c were attached as below. Relative expression of Vimentin and E-cadherin was calculated and normalized to the loading controls. The experiments were repeated for three times. The experiments were repeated for three times (**P < 0.001 vs. Control mice; ##P < 0.001 vs. db/db mice).