Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 17;11:3576. doi: 10.1038/s41467-020-17411-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Schematic of target DNA substrate. PAM site is orange; target sequence is blue; mismatches in the PAM distal region are red; gRNA is colored gray; cleavage sites are shown as red and blue vertical lines for the HNH and RuvC domains, respectively; tC^o labeled site at position −16 nt or −1 nt in the non-target strand is green. Cy3 and Cy5 labeled sites at position −6 nt and −16 nt in the target strand and non-target strand, respectively, are underlined. A time course of cleavage of on-target DNA (10 nM) was monitored with 28 nM of each enzyme. Data were fit to a double-exponential equation and the percentage of the total signal occurring with each decay rate is indicated. SpCas9 cleavage of on-target (a) (from Gong et al.¹⁵) and off-target (b) DNA. c, d HypaCas9 cleavage with on-target (c) and off-target (d) DNA. e, f Cas9-HF1 cleavage of on-target (e) and off-target (f) DNA. Data in f were fit to a single-exponential function.