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. 2020 Apr 27;14(8):1915–1928. doi: 10.1038/s41396-020-0648-9

Fig. 5. Effect of Bacillus on the growth and nodulation of rhizobia.

Fig. 5

a Bacillus colonies were inoculated next to a S. fredii CCBAU45436 colony at distances of 1.2 cm or 0.6 cm on a YMA plate. b Bacillus colonies were inoculated next to a B. diazoefficiens USDA110 colony at distances of 1.2 cm or 0.6 cm. c The growth phenotype of B-13 after contact with S. fredii CCBAU45436 in a. d The reverse colony of B. diazoefficiens USDA110 inoculated next to Bacillus B-9 in a. The results represent one of three replicates with similar results. The scale bar represents 2 mm. e The nodule numbers of CCBAU45436- or USDA110-inoculated plants treated with H2O or Bacillus under control conditions. f The nodule numbers of CCBAU45436- or USDA110-inoculated plants treated H2O or Bacillus under saline–alkali conditions (25 mM NaHCO3 + 75 mM NaCl); the experiment was repeated twice. Statistical analyses were performed by Mann–Whitney nonparametric tests and significance is denoted by asterisks where * indicates P < 0.05. Data are presented as median value ± SD (n = 4).