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. 2020 Apr 27;14(8):1915–1928. doi: 10.1038/s41396-020-0648-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Schematic representation of the mixed inoculation experiment, showing the planting, inoculation, and quantitative detection methods. Three-day-old plants were transplanted into control or saline–alkali-treated vermiculite, and then plants were inoculated with mixed rhizobia or mixed rhizobia containing Bacillus. Twenty-eight days later, the bacteroid DNA of the nodules was extracted, the concentrations of two kinds of rhizobia were quantified by qPCR, and the experiment was repeated twice. b The populations of Sinorhizobium or Bradyrhizobium bacteroids under control conditions. c The populations of Sinorhizobium or Bradyrhizobium bacteroids under saline–alkali conditions. Mix-R indicates six strains of rhizobium. qPCR results of the Mix-R and Mix-R + Bacillus treatments were analyzed with nonparametric Mann–Whitney tests (n = 4, ns nonsignificant; *P < 0.05). The horizontal bars of each graph indicate the median values and are listed where appropriate for clarity.