Fig. 3.

Absorption ELISA with incoA-tolerant and incoA-responsive sera. Sera from the third blood collection were subjected to absorption ELISA. ‘Un-absorbed’ denotes whole sera that were not depleted of antibodies, while ‘absorbed’ denotes patient sera that were depleted of antibodies against incoA before detecting anti-complexing protein hIgG by ELISA. Match-paired line graphs represent levels of hIgG against BoT/A and complexing proteins (un-absorbed) or only complexing proteins (absorbed) from incoA-tolerant patients (a) and incoA-responsive volunteers (b). *Statistical significance at p value < 0.05