Fig. 16.

Bar graphs show cortical microdialysate levels of adenine in the left and right kidneys during three experimental periods. After a 1-h stabilization period, microdialysate was collected for 45 min (period 1), and then the following drugs were added to the microdialysis perfusate of both kidneys: 10 μmol/L of erythro-9-(2-hydroxy-3-nonyl) adenine (blocks adenosine deaminase), 0.1 μmol/L of 5-iodotubericidin (blocks adenosine kinase), and 10 μmol/L of S-(4-nitrobenzyl)-6-thioinosine (blocks adenosine uptake). After 15 min for the drugs to reach the microdialysate probe tip, microdialysate was again collected for another 45 min (period 2). Next, in addition to the drugs listed above, adenosine (30 μmol/L) was added to the left kidney perfusate (period 3) and adenosine (30 μmol/L) plus forodesine (5 μmol/L; blocks purine nucleoside phosphorylase) was added to the right kidney perfusate (period 3). *P < 0.05, versus periods 1 and 2. Values are means ± SEM