Fig. 8.
Renal microvascular endothelial cells were treated with N6-etheno-ATP (10 μmol/L) for up to 8 h, and at the indicated time points, the medium was collected and analyzed for a N6-etheno-ATP, b N6-etheno-ADP, c N6-etheno-AMP, d N6-etheno-adenosine (ADO), and e N6-etheno-adenine (ADE) using reverse phase high-performance liquid chromatography as described in detail in the “Analytical methods” section. The sum (N6-etheno-Total) of N6-etheno-ATP, N6-etheno-ADP, N6-etheno-AMP, N6-etheno-adenosine, and N6-etheno-adenine is shown in f. In a, concentrations of N6-etheno-ATP were fitted to a mono-exponential decay function and the half-life (t1/2) was calculated. Values represent means and SDs. For some points, SD bars reside within the symbol. Individual data points are not shown because the symbols overlap