Table 3.

Determination of indices and frequencies

Items	Acute toxicity					Sub-chronic toxicity
	− 21 day	− 1 day	1 day	7 day	14 day	− 2 week	− 1 week	5 week	9 week	13 week	17 week
General symptoms	×	×	×	×	×	Once daily
Food consumption	×	×	×	×	×	×	×	Once weekly
Body weight	√	√	√	√	√	√	√	Once weekly
Temperature	√	√	√	√	√	√	√	√	√	√	√
	Before exposure and 30, 60 and 120 min post-exposure
Electrocardiography	√	√	√	√	√	√	√	√	√	√	√
	30, 60, and 120 min post-exposure
Ophthalmological examination	×	×	×	×	×	√	×	×	×	√	√
Fecal analysis	√	√	√	√	√	√	×	×	×	√	√
Urinalysis	√	√	√	√	√	√	√	√	√	√	√
Hematology	√	√	√	√	√	√	√	√	√	√	√
Serum chemistry	√	√	√	√	√	√	√	√	√	√	√
Bone marrow	×	×	×	×	×	×	×	×	×	√	√
Gross observation and necropsy	×	×	×	×	×	×	×	×	×	√	√
Histopathology	×	×	×	×	×	×	×	×	×	√	√

“√”: The item was evaluated at the planned time-point

“×”: The item was not evaluated