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. 2020 Jul 17;11:3607. doi: 10.1038/s41467-020-17444-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–c, 3D trajectory, intensity trace, and histogram of diffusion coefficients for single BSA molecules in 73.5 wt% glycerol (number of trajectories n = 43, bin siz = 0.5 μm²/s). d–g Tracking a range of dsDNA lengths at indicated glycerol concentrations. d 99 bp dsDNA tracking in 70 wt% glycerol-water solution. e 99 bp dsDNA tracking in 40 wt% glycerol. f 547 bps dsDNA tracking in 25 wt% glycerol. g 1385 bp dsDNA tracking in PBS solution. h, i 3D trajectory and intensity trace of DFHBI-1T labeled single Spinach RNA aptamers tracking in 75 wt% glycerol. j Stepwise bleaching is used to calculate the intensity of single DFHBI-1T dye. k The histogram of the number of DFHBI-1T for single Spinach RNA aptamers (number of trajectories n = 41, bin size = 1).