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. 2020 Jul 17;10:11876. doi: 10.1038/s41598-020-68516-y

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© The Author(s) 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Percentage and geometric mean fluorescence intensity of cell subpopulations positive for neutral GSL with terminal Gal residue. Notes Percentage of CSCs in the MDA-MB-231 and in the MDA-MB-453 (A) and non-CSCs in MDA-MB 231 and in the MDA-MB-453 cell lines (B). Geometric mean fluorescence intensity of CSCs in MDA-MB 231 and in the MDA-MB-453 (C) and non-CSCs in the MDA-MB-231 and in MDA-MB-453 cell lines after treatment with compound 1 in duration of 48 h. Data are expressed as a mean from experiment performed in triplicate ± SD. Columns, mean of viable cells; bars, SD; *P < 0.05; **P < 0.01. CSC MDA-MB-231, CD44⁺CD24⁻ cells of the MDA-MB-231 cell line; CSC MDA-MB-453, CD133⁺ cells of the MDA-MB-453 cell line; non-CSC MDA-MB-231, CD44⁺/CD24⁺, CD44⁻/CD24⁻ and CD44⁻/CD24⁺ cells of the MDA-MB-231 cell line; non-CSC MDA-MB-453, CD133⁻ cells of the MDA-MB-453 cell line; neolactotetraosylceramide or nLc₄Cer. GMI, geometric mean fluorescence intensity; SD, standard deviation.