Table 2.
CCD based experimental design and results.
| Run order | Peptone g/l | Chitin g/l | KH2PO4 g/l | MgSO4 g/l | Enzyme activity U/ml | |
|---|---|---|---|---|---|---|
| Experimental | Predicted | |||||
| 1 | 0.325 | 20 | 0.325 | 0.2 | 403 | 399.5833 | 
| 2 | 0.775 | 20 | 0.325 | 0.2 | 390 | 385.5417 | 
| 3 | 0.325 | 40 | 0.325 | 0.2 | 377 | 378.5417 | 
| 4 | 0.775 | 40 | 0.325 | 0.2 | 405 | 404.25 | 
| 5 | 0.325 | 20 | 0.775 | 0.2 | 383 | 383.2083 | 
| 6 | 0.775 | 20 | 0.775 | 0.2 | 388 | 388.9167 | 
| 7 | 0.325 | 40 | 0.775 | 0.2 | 397 | 394.9167 | 
| 8 | 0.775 | 40 | 0.775 | 0.2 | 437 | 440.375 | 
| 9 | 0.325 | 20 | 0.325 | 0.4 | 439 | 434.2083 | 
| 10 | 0.775 | 20 | 0.325 | 0.4 | 386 | 388.9167 | 
| 11 | 0.325 | 40 | 0.325 | 0.4 | 398 | 397.9167 | 
| 12 | 0.775 | 40 | 0.325 | 0.4 | 394 | 392.375 | 
| 13 | 0.325 | 20 | 0.775 | 0.4 | 352 | 353.5833 | 
| 14 | 0.775 | 20 | 0.775 | 0.4 | 331 | 328.0417 | 
| 15 | 0.325 | 40 | 0.775 | 0.4 | 347 | 350.0417 | 
| 16 | 0.775 | 40 | 0.775 | 0.4 | 360 | 364.25 | 
| 17 | 0.1 | 30 | 0.55 | 0.3 | 372 | 373.7083 | 
| 18 | 1 | 30 | 0.55 | 0.3 | 375 | 373.875 | 
| 19 | 0.55 | 10 | 0.55 | 0.3 | 373 | 377.7083 | 
| 20 | 0.55 | 50 | 0.55 | 0.3 | 397 | 392.875 | 
| 21 | 0.55 | 30 | 0.1 | 0.3 | 416 | 421.0417 | 
| 22 | 0.55 | 30 | 1 | 0.3 | 381 | 376.5417 | 
| 23 | 0.55 | 30 | 0.55 | 0.1 | 407 | 409.0417 | 
| 24 | 0.55 | 30 | 0.55 | 0.5 | 369 | 367.5417 | 
| 25 | 0.55 | 30 | 0.55 | 0.3 | 459 | 462 | 
| 26 | 0.55 | 30 | 0.55 | 0.3 | 463 | 462 | 
| 27 | 0.55 | 30 | 0.55 | 0.3 | 466 | 462 | 
| 28 | 0.55 | 30 | 0.55 | 0.3 | 462 | 462 | 
| 29 | 0.55 | 30 | 0.55 | 0.3 | 459 | 462 | 
| 30 | 0.55 | 30 | 0.55 | 0.3 | 467 | 462 | 
| 31 | 0.55 | 30 | 0.55 | 0.3 | 458 | 462 |