Fig. 3. Nebivolol increases the amount of IF1 bound to the ATP synthase.

HCT116 cells were treated (NEB, red) or not (CRL, black) during 3 h with 1 µM nebivolol. a Representative BN-immunoblots of mitochondrial membrane proteins blotted with the antibodies of the indicated subunits of the different OXPHOS complexes. The migration of supercomplexes (SC); complex I and I + III₂ (NDUFA9); complex II (SDHB); complex III₂, III₂ + IV and I + III₂ (Core 2); complex IV, IV₂ and III₂ + IV (COX IV); oligomers (Vn), dimers (V₂), monomers (V), F1-ATPase and β-F1-ATPase subunit of the ATP synthase and their co-migration with IF1 are indicated. The migration of molecular mass markers is indicated in the Coomassie-stained gel and applied for all blots. VDAC and Coomassie-stained gel are shown as loading controls. b Plots of the quantification of the relative migration (arrowhead) of fractionated OXPHOS complexes. Bars indicate the mean of three different experiments ±SEM. *p = 0.042 (V) and 0.039 (F1) when compared to CRL by two-sided Student’s t test. Source data are provided as a Source Data file.