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. 2020 Jul 17;3:389. doi: 10.1038/s42003-020-1101-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Mitochondria were analyzed for cristae connectivity. a Top panel: individual slices from SBF–SEM imaging showing cristae networking. Middle panel: cross-section of composite stacks indicating networked (yellow) and orphaned cristae (red). Bottom panel: mitochondrial cross-section showing only networked cristae (from middle panel). Composition of total mitochondrial volume (b), total cristae volume (c), and connected cristae volume (d) were measured in each experimental groups. N = 10 for all groups. Scal bar represents 250 nm. *P < 0.05 versus normoxic; ^#P < 0.05 versus I/R.