Table 1.

Number of sequence reads detected for each of the spiked viruses at the different spiking levels in the viral vaccine crude harvest.

Virus	Spiking level (GC mL−1)
	105	104	103	103	102	102
Total raw reads	291,341,932	371,184,528	365,131,932	333,373,964	437,070,286	332,181,730
NIH
Adenoviridae
Adenovirus 5	14,382	2284	264	193	34	34
Adenovirus 41	1154	164	12	16	11	6
Flaviviridae
Bovine Viral Diarrhea Virus	95,666	6306	776	926	68	57
Herpesviridae
Herpes Simplex Virus Type 1	1366	200	40	23	6	2
Simian Cytomegalovirus	49,266	9386	1170	676	116	87
Orthomyxoviridae
Influenza A Virus	16,553	1266	83	166	8	3
Paramyxoviridae
Bovine Parainfluenza Virus Type 3	48,578	3548	321	410	24	32
Measles Morbillivirus	12,571	922	87	105	0	10
Mumps Virus	9041	524	86	46	10	2
Picornaviridae
Coxsackievirus A16	351	27	1	0	3*	0
Coxsackievirus B3	2036	157	10	18	9	8
Echovirus 11	2539	140	4	14	5*	1
Rhinovirus 2	1259	62	6	20	0	4
Polyomaviridae
Simian Virus 40	528	88	47	15	10	3*
Rhabdoviridae
Vesicular Stomatitis Virus	13,120	1071	102	157	27	12
Togaviridae
Rubella Virus	4610	391	76	51	4	2
SP
Bornaviridae
Human Borna Disease Virus	341	6	1	0	0	0
Coronaviridae
Bovine Coronavirus	7447	473	48	46	5*	2
Herpesviridae
Human Cytomegalovirus	162,008	28,013	2733	2657	305	252
Parvoviridae
Minute Virus of Mice	319	48	12	10	0	0
Porcine Parvovirus	146,720	20,668	2412	2609	397	238
Reoviridae
Reovirus Type 3	11	0	0	0	0	0

Replicate spikes were made at 10³ and 10² GC/mL as it was hypothesized that the LOD may be near this spike level.

GC genome copy, NIH National Institutes of Health, SP Sanofi Pasteur.

*3 or more reads belonging to one contig that is less than 201 bp (does not meet criteria for positive result).