Table 3.
Number of sequence reads detected for each of the spiked viruses at the different spiking levels in the cell substrate matrix.
| Virus | Genome copies per cell | |||
|---|---|---|---|---|
| 0.01 | 0.01 | 0.001 | 0.001 | |
| NIH | ||||
| Adenoviridae | ||||
| Adenovirus 5 | 244 | 125 | 18 | 4 |
| Adenovirus 41 | 21 | 8 | 0 | 0 |
| Flaviviridae | ||||
| Bovine Viral Diarrhea Virus | 1014 | 1096 | 11 | 12 |
| Herpesviridae | ||||
| Herpes Simplex Virus Type 1 | 58 | 17 | 0 | 0 |
| Simian Cytomegalovirus | 449 | 386 | 2 | 32 |
| Orthomyxoviridae | ||||
| Influenza A Virus | 0* | 0* | 0* | 0* |
| Paramyxoviridae | ||||
| Bovine Parainfluenza Virus Type 3 | 627 | 512 | 25 | 11 |
| Measles Morbillivirus | 43 | 45 | 0 | 0 |
| Mumps Virus | 181 | 253 | 2 | 4 |
| Picornaviridae | ||||
| Coxsackievirus A16 | 10 | 9 | 0 | 0 |
| Coxsackievirus B3 | 55 | 106 | 0 | 0 |
| Echovirus 11 | 110 | 86 | 0 | 0 |
| Rhinovirus 2 | 6 | 20 | 0 | 0 |
| Polyomaviridae | ||||
| Simian Virus 40 | 48 | 29 | 1 | 0 |
| Rhabdoviridae | ||||
| Vesicular Stomatitis Virus | 580 | 674 | 5 | 7 |
| Togaviridae | ||||
| Rubella Virus | 130 | 41 | 0 | 0 |
| SP | ||||
| Bornaviridae | ||||
| Human Borna Disease Virus | 14 | 10 | 0 | 9 |
| Coronaviridae | ||||
| Bovine Coronavirus | 35 | 41 | 0 | 2 |
| Herpesviridae | ||||
| Human Cytomegalovirus | 12,990 | 13,089 | 554 | 551 |
| Parvoviridae | ||||
| Minute Virus of Mice | 0* | 14* | 0* | 0* |
| Porcine Parvovirus | 5428 | 4932 | 327 | 214 |
| Reoviridae | ||||
| Reovirus Type 3 | 0* | 0* | 0* | 0* |
Replicate spikes were made at each spiking level.
GC genome copy.
*indicates that the LOD for the specific virus was not identified through this study.