Figure 1.

Simultaneous analysis of platelet deposition, thrombus phenotype and fibrin formation during whole blood thrombus formation under flow. Citrated whole blood from control subjects (n = 5–10) was supplemented with fluorescent labels to simultaneously detect platelet adhesion (DiOC₆), procoagulant, phosphatidylserine (PtdS)-exposing platelets (AF568-annexin A5) and fibrin formation (AF647-fibrinogen). Blood samples were co-perfused with CaCl₂/MgCl₂ over indicated microspots, at a shear rate of 1,000 s^-1. Microspot coding: M1, blocking buffer BSA; M2, rhodocytin + VWF; M3, laminin + VWF; M4, collagen-III; M5, collagen-I low; M6, collagen-I high; M7, GFOGER-GPO + VWF-BP; co-coating with 500 pM TF as indicated. (A) Representative bright-field and fluorescence images,

taken from thrombi on microspots without TF (6 min). (B) Idem, from thrombi on microspots co-coated with 500 pM TF (6 min). Bars = 20 µm.