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. 2020 Jul 17;10:11910. doi: 10.1038/s41598-020-68438-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alterations in whole blood thrombus formation by immobilized thrombomodulin or activated protein C (APC). (A–E) Collagen-I microspots were co-coated or not with TM (M8) or APC (M9), and subjected to whole-blood flow at 1,000 s^-1 (TF in recalcification medium). (A) Representative bright-field and fluorescence microscopic images, as in Fig. 1, taken after 6 min; bars = 20 µm. (B–D) Platelet deposition (B), phosphatidylserine (C) and fibrin formation (D) after 6 min of flow. (E), Time to fibrin formation per microspotted surface, demonstrating delaying effects of TM and APC. Means ± SEM. n = 10–11, *p < 0.05. **p < 0.01, ***p < 0.001. (F,G) C4BP or soluble APC (sAPC) were added to whole blood before flow perfusion over collagen-I microspots co-coated or not with TF (M6). Time to fibrin formation (n = 4–7) in the presence of C4BP (F) or sAPC (G).