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. 2020 Jul 17;10:11910. doi: 10.1038/s41598-020-68438-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Altered thrombus and fibrin formation in patients with coagulation factor abnormalities. Blood samples from healthy day control subjects (n = 10) and patients (Pat, n = 8) with indicated coagulation factor abnormalities were investigated for platelet, thrombus, and fibrin formation at a shear rate of 1,000 s^-1, with image capturing every 2 min (see Fig. 2). Image parameters (P1–9) were obtained for microspot M6 no TF (rows A) or with TF (rows B); M7 no TF (rows C) or with TF (rows D); M8 with TF (rows E). Data were univariate scaled (0–10) across surfaces per parameter for all flow runs. Differential heatmap of scaled parameters over time per patient in comparison to means of control subjects. Filtering applied for changes outside the range of means ± SD of control subjects. A green colour indicates a relevant decrease, a red colour a relevant increase in comparison to controls. Full data are given in Data File 5.