Table 1.
Coding of microspots (M) and parameters (P) in whole blood thrombus formation, and curve characteristics (C) of thrombin generation.
| M | 1st Spot | Receptors | 2nd Spot | Pathway |
|---|---|---|---|---|
| Co-coating TF | ||||
| M1 | Control BSA | None | + TF | FVIIa |
| M2 | Rhodocytin + VWF | GPIb, CLEC2 | + TF | FVIIa |
| M3 | Laminin + VWF | GPIb, α6β1 | + TF | FVIIa |
| M4 | Collagen-III (VWF)a | GPIb, VI, α2β1 | + TF | FVIIa |
| M5 | Collagen-I low (VWF)a | GPIb, VI, α2β1 | + TF | FVIIa |
| M6 | Collagen-I high (VWF)a | GPIb, VI, α2β1 | + TF | FVIIa |
| M7 | GFOGER-(GPO)n + VWF-BP | GPIb, VI, α2β1 | + TF | FVIIa |
| Co-coating anticoagulant | ||||
| M8 | Collagen-I + TM | GPIb, VI, α2β1 | − TM | Protein C |
| M9 | Collagen-I + APC | GPIb, VI, α2β1 | − APC | Protein C |
| P | Time (min) | Image type | Description | Unit |
|---|---|---|---|---|
| Platelet parameters | ||||
| P1 | 0–8 | DiOC6 | Platelet adhesion | %SAC |
| P2 | 0–8 | AF568-annexin A5 | Platelet PtdS exposure | %SAC |
| Thrombus parameters | ||||
| P3 | 0–8 | Bright-field | Thrombus coverage | %SAC |
| P4 | 0–8 | Bright-field | Thrombus morphology score | 1–5 |
| P5 | 0–8 | Bright-field | Thrombus aggregation score | 1–3 |
| P6 | 0–8 | Bright-field | Thrombus contraction score | 1–3 |
| Fibrin parameters | ||||
| P7 | 0–8 | AF647-fibrin(ogen) | Fibrin deposition | %SAC |
| P8 | 0–8 | Bright-field | Fibrin score | 1–3 |
| P9 | 0–8 (10) | AF647-fibrin | Shorter time to fibrin | 11—t min |
| C | Curve characteristic | Unit | ||
|---|---|---|---|---|
| C1 | Shorter time to first thrombin | min | ||
| C2 | Endogenous thrombin potential (ETP) | nM min | ||
| C3 | Thrombin peak | nM | ||
1st Spots were located at upstream positions. Numbering of variables according to appearance in heatmaps.
aBinding VWF from plasma. PtdS phosphatidylserine.